• IVIS = In Vivo Imaging System

  • Uses the full wavelength spectrum from high energy blue to low energy infrared (430 – 850 nm)

    • Bioluminescent reporters (light emitted following chemical reaction of the luciferase enzyme with its substrate)

    • Fluorescent reporters 

    • Ideal for distinguishing multiple bioluminescent and fluorescent reporters

  • Combines 2D optical and 3D optical tomography

  • Allows for distinguishing different organs in a high-resolution (to 20 microns)

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• Two key areas

  • Anatomical/morphological imaging

  • Molecular imaging

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• Facilitates small animal (whole body) noninvasive longitudinal monitoring of disease progression, cell trafficking, and gene expression patterns in living animals

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Facilitates terminal ex vivo tissue imaging from small and large animals